There is no correlation between serum caspase-3 levels and the systemic levels of reactive oxygen species in septic patients

SEBASTIAN P. CHAPELA , HILDA ISABEL BURGOS, AMALIA SCHIEL , PABLO LUCERO , FLORENCIA MARÍA BALLESTERO , MIGUEL ANGEL BLASCO , MANUEL ALONSO , CARLOS ALBERTO STELLA

Congreso; 13th Worls Congress of Intensive & Critical Care Medicine. WFSICCM; 2017

Objectives: To evaluate the correlation between serum markeractivated Caspase-3 and levels of reactive oxygen species (ROS).Methods: Prospective observational study. 55 septic patients admittedto the intensive care unit and 24 healthy volunteers were included. Aserum sample was taken at the time of admission, and activatedCaspase-3 and ROS were measured using the ELISA method and theDichlorofluorescein-Diacetate (DCFH) technique, respectively. Thelevels of healthy controls were compared with those of septic patientsand septic patients who survived and died.Results: Septic patients had higher levels of Caspase-3 than controls(34.16 pg/mL vs 2.1 pg/mL) (P b .001), with no difference in DCFHemission levels (0.1 vs 0.109) (P = .36). Patients who died hadhigher levels of Caspase-3 than those who survived (51.45 pg/mL vs25.7 pg/mL) (P = .007), and there was no difference in DCFH levels(0.099 vs 0.103) (P = .68) or correlation between DCFH andactivated Caspase-3 levels (rho = 0.068; P = .62).Conclusion: It has been observed in this study that septic patientsand patients who died had higher activated Caspase-3 serum levels,being able to use this protein as a marker of mortality in the ICU.There were no differences in DCFH levels among any group. Therewas also no correlation between DCFH and activated Caspase-3levels, which suggests that the apoptosis and ROS generationprocesses are not related.https://doi.org/10.1016/j.jcrc.2017.09.078