Effect of Dichloroacetate on S.cerevisiae resistance to fluconazole

CONGOST, C.A.,CHAPELA SP, BURGOS, H.I., ALONSO M., STELLA, C.A.

Córdoba

Congreso; 52th Reunión Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2016

SAIB

Effect of Dichloroacetate on S.cerevisiae resistance to fluconazoleResearches with yeast as a model system have provided information concerning a variety of cellular metabolic processes. Metabolic similarities between mammalian cells and yeast make possible the use of these latter for elucidating the effect of drugs in similar situations. Compound dichloroacetate (DCA) has been used to treat congenital lactic acidosis. Considering the capacity of acidification of yeast in fermentation conditions, this work attempts to analyze: 1) What effect exerts the DCA on the ability of S. cerevisiae for acidification? and 2) What result has the phenotype obtained on antifungal fluconazole resistance, by growing yeast in the presence of DCA? Taking into account these objectives, we determined, in rich medium (1% yeast extract, 1% peptone and 2% glucose), the effect of DCA on the external acidification of yeast. Subsequently, the antifungal resistance was tested. The results show that the DCA (3.0 mg / ml) decreases external acidification produced by yeast and sensitizes the cell to growth inhibition by fluconazole. Increased sensitivity of yeast against crystal violet compound is also observed.