INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Nitric Oxide-Sensitive Guanylyl Cyclase alpha1 and beta1 Subunits Are Differentially Affected By Low Doses of Cadmium and Arsenic in Anterior Pituitary Gland and Uterus In Vivo and in Vitro
Autor/es:
BENZO YANINA; RONCHETTI SONIA; CABILLA JIMENA; DUVILASKI BEATRIZ
Lugar:
Boston, Massachusetts
Reunión:
Congreso; The Endocrine Society 98th Annual Meeting and Expo. Endo 2016; 2016
Institución organizadora:
Endocrine Society
Resumen:
17β-estradiol (E2) is the major female sex hormone, mediating the differentiation, proliferation, development and homeostasis of female reproductive functions. Cadmium (Cd) and arsenic (As) are environmental pollutants that at low dose mimic E2 effects on hormone-dependent tissues. The main nitric oxide receptor, nitric oxide-sensitive or soluble guanylyl cyclase (sGC), is a heterodimer composed by two subunits α and β and catalyzes cGMP formation. Previously we have shown that E2 exerts opposite effects on these sGC subunits, increasing both α1 mRNA and protein levels and decreasing β1 expression in vitro and in vivo. Besides, α1 increase has been strongly associated with cell proliferation and tumor progression in androgen-dependent tumor cell line LNCaP. The aim of the present work was to investigate the xenoestrogenic effect of Cd and As on sGC subunits expression in the lactosomatotroph cell line GH3, in anterior pituitary gland and uterus.In vitro, 1 nM Cd and 1 nM As treatments stimulates GH3 cell proliferation after 72 h (% of BrdU incorporation; control: 23.8±2.2; Cd: 45.5±2.7**; As: 51±3**; E2: 50±4**, p