INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Involvement of nitric oxide and TNF-alpha in cell death and proliferation of GC-1 spermatogonia cell line
Autor/es:
FERREIRO MARÍA EUGENIA; GONZÁLEZ CANDELA; THEAS MARÍA SUSANA; SOBARZO CRISTIAN; LUSTIG LIVIA; MÉNDEZ SOLEDAD ; JACOBO PATRICIA
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2016
Institución organizadora:
SAIC-SAI-SAFE
Resumen:
Nitric oxide (NO) and Tumor Necrosis Factor alpha (TNF-α) are pleiotrophic factors that display multiple biological functions such as death induction and cell cycle control. In experimental autoimmune orchitis (EAO) a rat model of chronic inflammation associated to infertility, spermatocytes die by apoptosis induced by high levels of NO and TNF-a while the number of proliferating spermatogonia is reduced. We propose that these factors are also involved in spermatogonia cell cycle control. To confirm this hypothesis we explore the role of NO and TNF-α/TNFR system of the mouse immortalized spermatogonia (GC-1) proliferation and death. DETA-Nonoate (DETA, a NO donor) reduced GC-1 viability evaluated by the colorimetric assay of MTS (mean±SEM, media: 0.499±0.013; DETA 2mM:0.344±0.024**; DETA 0.5mM:0.522±0.019; DETA 0.05mM:0.580±0.033 **p