Humanin increases the resistance of breast cancer cells to chemotherapy

M.F.GOTTARDO; M.IMSEN; A.ASAD; V.ROMANOWSKI; M.CANDOLFI; M.MORENO AYALA; M.PIDRE; A. SEILICOVICH

Mar del Plata

Congreso; LXI Reunión de la Sociedad Argentina de Investigación Clínica; 2016

Humanin (HN) is a mitochondrial derived peptide with potent cytoprotective action in many cell types. It has been described that endogenous and exogenous HN can protect normal tissues against toxic effects of chemotherapy. However, the role of this peptide in tumor development is not well known. We have previously shown that HN is expressed in murine mammary carcinoma cells (4T1) and exerts antiapoptotic and mitogenic actions against various insults such as serum deprivation or TNF-α. The aim of this study was to investigate whether HN affects the sensitivity of tumor cells to chemotherapeutic agents. HN (10 µM) hampered the antiproliferative effect of doxorubicin (DOXO, 500 nM) on 4T1 cells in vitro (by ELISA BrdU; C=0.55±0.04; DOXO=0.34±0.02*, HN=0.52±0.02, DOXO+HN=0.53±0.01, *p