INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Analysis of typical and atypical MAPK phosphatases (MKPs) expression in the aggressive phenotype of breast cancer cells
Autor/es:
CASTILLO AF; PAZ C; MORI SEQUEIROS GARCIA MM; COHEN SABBAN JM; ORLANDO U; MALOBERTI PM
Lugar:
Mar del Plata, Buenos Aires, Argentina
Reunión:
Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2016
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
MKPs are a heterogeneous group of dual-specificity phosphatases (DUSP) that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues. This group of enzymes includes typical MKPs (such as MKP-1, 2 and 3), which specifically dephosphorylate members of the MAPK family, and atypical MKPs (such as DUSP15, 18, 22), much less characterized than the first group. A large number of phosphatases are involved in tumor development, pointing to a central role of this group of enzymes in the regulation of proliferation and cell differentiation. The expression of acyl-CoA synthetase 4 (ACSL4), an enzyme that participates in arachidonic acid metabolism, has been associated with more aggressive forms of several types of cancer. ACSL4 is part of the mechanism responsible for increased breast cancer cell proliferation, invasion and migration. Thus, the aim of this work was to analyze the expression of a typical, MKP-3, and an atypical MKP, DUSP18, in cells with different ACSL4 expression levels. The experimental model is based on the stable transfection of MCF-7 cells with ACSL4 using the tetracycline Tet-Off system (MCF-7 Tet-Off/ACSL4), in which doxycycline down-regulates ACSL4 expression. mRNA levels of MKP-3 and DUSP18 were evaluated by semiquantitative RT-PCR. The expression of MKP-3 and DUSP18 was increased 1.5 and 1.8-fold (P