INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Modulation of pituitary cell renewal by L-3,4-dihydroxyphenylalanine (L-Dopa): role of its conversion to dopamine (DA)
Autor/es:
MARIELA MORENO AYALA; JIMENA FERRARIS; SANTIAGO JORDI ORRILLO; SANDRA ZÁRATE; DANIEL PISERA; NATALY DE DIOS; FLORENCIA GOTTARDO
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Congreso; 2nd FALAN Congress; 2016
Resumen:
L-Dopa plus peripheral aromatic L-amino acid decarboxylase (AADC) inhibitors is the gold standard treatment for Parkinson?s disease. L-Dopa crosses the blood brain barrier and is converted to DA by AADC. Since DA induces apoptosis in anterior pituitary, our hypothesis is that L-Dopa modulates the pituitary cell turnover by its conversion to DA. First, the expression of AADC was studied by immunofluorescence. We observed in pituitary sections that the enzyme is expressed in the anterior lobe. Likewise, GH3 and AtT20 pituitary cell lines were immunoreactive for AADC. These results were confirmed by western blot. To study the effects of L-Dopa on cell renewal, GH3 cells were incubated with L-Dopa (1uM, 8 h) in the presence of an AADC inhibitor (NSD 1015, 1uM). Apoptosis and proliferation were determined by TUNEL and BrdU incorporation, respectively. L-Dopa increased the percentage of TUNEL-positive cells, but the concomitant inhibition of AADC revealed an antiapoptotic effect of L-Dopa (C: 13.65; L-Dopa: 22.73; NSD: 14.50; L-Dopa+NSD: 7.57, p