Embryo cardio-placental anomaly in murine organogenesis following maternal alcohol consumption. Effects on the VEGF/receptors system

C. SOBARZO; VENTUREIRA MARTIN; CEBRAL ELISA; CALVO JUAN CARLOS

Buenos Aires

Congreso; XVIII Jornadas Anuales Multidisciplinarias de la Sociedad Argentina de Biología (SAB; 2016

Sociedad Argentina de Biología

Maternal alcohol consumption causes the fetal alcohol syndrome, characterized by various congenital diseases. Previously, we saw delayed embryo growth and increased dysmorphogenesis after perigestational 10% alcohol treatment up to murine organogenesis. Since embryo-fetal cardiogenesis is one of the targets of maternal alcohol intake and cardiac abnormalities may be closely related to altered labyrinthine vascular development due to imbalances in the VEGF/receptors system, the objectives were to analyze, the organogenic embryo cardiac histopathology (H&E), the labyrinthine histo-morphology and growth (H&E, nuclear Hoechst fluorescent staining, Image Pro Plus), the proliferation (PCNA immunohistochemistry (IHC), the apoptotic index (TUNEL) and VEGF, KDR and Flt-1 expressions (IHC and western blot (WB) in the embryo-trophoblast tissues. Ethanol 10%/drinking water was administered to murine CF-1 females for 15 days before and until day 10 of gestation (TF) (Control females (CF) without ethanol). TF had elevated % of embryos with irregulardiscontinuous endocardium (p