INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of ERK /2 in the differentiation of primary cultures of dental pulp cells
Autor/es:
DE COUTO PITA, A K; MENDEZ, C F; MERHAR, V; PAZ, C; ACQUIER, A B
Lugar:
Mar
Reunión:
Congreso; 49º Reunión científica anual de la Sociedad Argentina de Investigación Odontológica (SAIO); 2016
Institución organizadora:
Sociedad Argentina de Investigación Odontológica (SAIO)
Resumen:
Previously, we reported the establishment of primary culture human dental pulp cells (HDPCs) that exhibit morphology changes in the presence of osteoblastic differentiation factors. The extracellular signal kinase type 1 and 2, ERK 1 /2 (ERK) is activated by a variety of receptors implicated in growth and differentiation process and thus regulates numerous cellular functions.Objective: The aim of the present study was to investigate whether the addition of differentiation conditioning factors modifies the cell proliferation rate and to determine the possible participation of ERK in the beginning of the differentiation process towards a mineral-producing cell type.Materials and Methods: Cultures were established by cell explant from mechanically disaggregated pulp after removal of retained molars (n=2) extracted from adult donors due to eruption failures. The tissue was incubated at 37°C and 5% CO2 in Eagle's modified Dulbecco's medium (DMEM) with 10% fetal bovine serum (FBS) and L-glutamine or in DMEM supplemented with 300 µM ascorbic acid, 10 mM β glycerophosphate, 15% FBS and 0.5 mM dexamethasone as differentiation factors (DMEM-OD). Cell proliferation was colorimetrically analyzed after crystal violet staining and ERK activation was determined through the detection of the phosphorylated form of ERK 1/2 (p-ERK) by Western blot using specific antibodies.Results: In culture, cells proliferated in DMEM medium up to day 7 with a doubling time of 24 hrs, whereas incubation in DMEM-OD medium resulted in a significant reduction in proliferation (p