INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Nitric oxide-sensitive guanylyl cyclase 1 and 1 subunits are differentially affected by low doses of cadmium and arsenic in anterior pituitary gland and uterus in vivo and in vitro.
Autor/es:
CABILLA JIMENA P; DUVILANSKI BEATRIZ H; RONCHETTI SONIA A; BENZO Y
Lugar:
Boston
Reunión:
Congreso; 98th Annual Meeting of The Endocrine Society; 2016
Resumen:
17b-estradiol (E2) is the major female sex hormone, mediating the differentiation, proliferation, development and homeostasis of female reproductive functions. Cadmium (Cd) and arsenic (As) are environmental pollutants that at low dose mimic E2 effects on hormone-dependent tissues. The main nitric oxide receptor, nitric oxide-sensitive or soluble guanylyl cyclase (sGC), is a heterodimer composed by two subunits a and b and catalyzes cGMP formation. Previously we have shown that E2 exerts opposite effects on these sGC subunits, increasing both a1 mRNA and protein levels and decreasing b1 expression in vitro and in vivo. Besides, a1 increase has been strongly associated with cell proliferation and tumor progression in androgen-dependent tumor cell line LNCaP. The aim of the present work was to investigate the xenoestrogenic effect of Cd and As on sGC subunits expression in the lactosomatotroph cell line GH3, in anterior pituitary gland and uterus.In vitro, 1 nM Cd and 1 nM As treatments stimulates GH3 cell proliferation after 72 h (% of BrdU incorporation; control: 23.8±2.2; Cd: 45.5±2.7**; As: 51±3**; E2: 50±4**, p