INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LONG-TERM OVARIAN HORMONE DEPRIVATION INDUCES MITOCHONDRIAL DYSFUNCTION AND CHANGES IN MITOCHONDRIAL MEMBRANE LIPID PROFILE IN THE HIPPOCAMPUS
Autor/es:
ZARATE SANDRA; ASTIZ MARIANA; MAGNANI NATALIA; IMSEN MERCEDES; MERINO FLORENCIA; ALVAREZ SILVIA; REINES ANALIA; SEILICOVICH ADRIANA
Lugar:
Rio de Janeiro
Reunión:
Congreso; 9° World Congress International Brain Research Organization; 2015
Institución organizadora:
IBRO (International Brain Research Organization)
Resumen:
Mitochondrial dysfunction is involved in age-related disorders. During reproductive senescence, there is a gradual loss of ovarian hormones, which exert neuroprotective actions increasing mitochondrial respiratory efficiency and functions. Also, mitochondria functional integrity is highly dependent on membrane fatty acid and phospholipid composition, mainly cardiolipin content and its acyl chain composition, parameters that are also affected during aging. The aim of the present work was to study the effect of long-term ovarian hormone deprivation upon mitochondrial function and its putative correlation with changes in mitochondrial membrane lipid profile in the hippocampus, one of the areas primarily affected in age-related disorders and highly responsive to ovarian hormones. To this aim, Wistar adult female rats were ovariectomized or sham-operated and after 12 weeks we evaluated different parameters of mitochondrial function as well as membrane fatty acid composition and phospholipid content in mitochondria isolated from the hippoccampus. Mitochondria from OVX rats showed, compared with SHAM rats, a marked decrease in the rates of active (state 3) respiration (SHAM: 68 ± 4, OVX: 58 ± 6 ng-at O/min.mg protein; p