INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NATURAL ANTISENSE TRANSCRIPTS (NATS): FUNCTIONAL ROLE ON ACYL-COA SYNTHETASE 4 (ACSL4) EXPRESSION
Autor/es:
CASTILLO, ANA FERNANDA; ORLANDO, ULISES; MALOBERTI, PAULA; PODESTA, ERNESTO J
Lugar:
Buenos Aires
Reunión:
Congreso; Molecular mechanisms in cell signaling and gene expression Argentine Society for Biochemistry and Molecular Biology scientific meeting. SAIB 2013.; 2013
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
The acyl-CoA synthetase 4 (Acsl4), an enzyme involved in arachidonic acid metabolism, is crucial in steroidogenesis and in pathologic processes such as tumorigenesis Acsl4 is rapidly induced in response to hormones and has a short half-life; however the mechanisms regulating its expression are not fully described. NATs are endogenous RNAs complementary to mRNA, which act on transcription, maturation, transport, stability or translation of its counterparts. We have identified an Acsl4 NAT which is a long non-coding RNA complementary to an Acsl4 mRNA alternative splicing variant. The aim of this study was to evaluate the physiological role of Acsl4 NAT in the mechanism of Acsl4 expression and action. Hormonal regulation of this NAT was assessed. Sequence-specific RT-PCR was performed on MA-10 Leydig cells treated with gonadotropin. Hormone and cAMP treatments increase its expression in a time-dependent manner, reaching a maximum level after 5 h of stimulation. Acsl4 NAT overexpression impacts differentially on Acsl4 transcripts levels, increasing mRNA var 2 expression, protein synthesis and steroidogenesis. Acsl4 NAT knockdown via asymmetric shRNA showed opposite effects. Acsl4 NAT is one of the few described NATs that are hormonally regulated. Therefore, Acsl4 NAT is a new player in the regulation of Acsl4 mRNA expression and activity, thus influencing steroidogenesis regulation.