LOCAL THERAPY FOR ORCHITIS-INDUCED INFERTILITY IN RATS: MELATONIN WITHIN THERMOSENSITIVE NANOPLATFORMS

MUNDURUCA PIRES THAISY; OCAMPO CAROLINA; SOBARZO CRISTIAN MARCELO,; FERRER VIÑALS DENISSE; GONZÁLEZ LUCAS; JACOBO PATRICIA; THEAS MARIA SUSANA; MAIO MARIA BELEN; GLIENKE LEILANE; LUSTIG LIVIA; GLISONI ROMINA

CABA

Congreso; CONGRESO CONJUNTO MAESTRIA EN CS BIOMEDICAS FFyB- UNIVERSIDAD de FRIBURGO ALEMANIA; 2023

FFYB, UBA7 UNIVERSIDAD de FRIBURGO ALEMANIA

Approximately 30-50% of cases of infertility are due to a male factor; a reduction in the quality of sperm in which oxidative stress plays a significant role.Experimental autoimmune epididymo-orchitis (EAEO) is a well-established rodent model for studying the impact of inflammatory processes and oxidative stress on spermatogenesis associated with infertility. Melatonin (MLT) is an antioxidant and antiapoptotic hormone, and its properties can be harnessed for counteracting the EAEO inflammatory environment. However, it has limited solubility in aqueous medium and short half-life.Pluronic® F127 is a biocompatible nanoplatform of polymeric micelles (PMs) based of PEO-PPO block copolymers exhibiting reverse thermal gelation at body temperature and a at concentration higher of 20% (w/v) in biological medium, used for enhancing drug delivery and solubility.Our aim was to optimize melatonin delivery and improve its availability within the testes, employing Pluronic® F127, thereby exploring its capacity to counteract the impact of oxidative stress on spermatogenesis and sperm quality in EAEO.Melatonin-F127 25% injected into the testis had a positive effect on stored epididymal sperm through its strong antioxidant properties and also a reduction in inflammatory cells.Our study suggested that local melatonin delivery through thermosensitive polymeric micelles, might be a good strategy to address orchitis-induced male infertility.