Evaluation of Mitochondrial DNA Mass in Women with Polycystic Ovary Syndrome

VELAZQUEZ MARIELA EDITH; MOTTA ALICIA; MAILÉN ROJO; MARÍA CONSTANZA PAUTASSO; CERRONE GLORIA EDITH; ANDREA LILIANA MILLÁN; ABRUZZESSE GISSELLE

Buenos Aires

Congreso; Reunión anual de Sociedades de Biociencia; 2020

ObjectivePolycystic ovary syndrome (PCOS) is characterized by insulin resistance (RI) which can influence the content of mitochondrial DNA (mtDNA). Our objective was to evaluate the content of mtDNA in women with PCOS compared with control women and the relation with metabolic parameters.Materials and methods:We studied fifty women with PCOS and thirty-four control women aged 17 to 45 years. The determination of the number of copies of mtDNA was carried out in peripheral blood leukocytes by Quantitative real-time PCR, SYBR Green method. Statistical analysis were carried out by Student´s t-test, correlation and linear regressions with a significance level of 0.05 (SPSS 25).Results:Compared to controls, PCOS patients have higher weight, body mass index (BMI), waist circumference (WC), also higher levels of TG, LDL, total cholesterol, fasting plasma glucose and lower levels of HDL cholesterol. By linear regression we observed that mtDNA was significantly lower in the presence of PCOS (135.57 ± 81.34 vs 190.37 ± 135.19; p = 0.023). A significant negative correlation was observed between the mtDNA content and telomere length (p = 0.034). Within PCOS patients, women with insulin resistance according to a HOMA index> 2.5 have a lower mtDNA content compared to PCOS women with HOMA