BYSTANDER ACTIVATION OF MICROGLIA BY BRUCELLA ABORTUS-INFECTED NEUROVASCULAR CELLS INDUCES NEURONAL DEATH

RODRÍGUEZ J; GIAMBARTOLOMEI, GUILLERMO H; DELPINO MV; ANA MARÍA RODRIGUEZ

Buenos aires

Congreso; Reunión anual de la Sociedad Argentina de Inmunología (SAI). Formato Virtual 2020.; 2020

SAIC, SAI, SAFIS

Central nervous system invasion by bacteria of the genus Brucellaresults in an inflammatory disorder called neurobrucellosis. We havepreviously demonstrated that B. abortus is able to elicit neuronaldeath by activating microglia (with release of nitric oxide and proinflammatorycytokines) and inducing primary phagocytosis. The aimof this work was to investigate the role of soluble mediators releasedby infected cells of the neurovascular unit in the modulation of thisphenomenon. For this, murine astrocytes and human brain microvasculatureendothelial cells (HBMECs) were infected or not with B.abortus for 24h. After that, cell free culture supernatants were usedto stimulate co-cultures of murine primary neuron/microglia during48h. Neuronal density was evaluated by fluorescence microscopy.Stimulation of neuron/microglia co-cultures with supernatants fromB. abortus-stimulated astrocytes or HBMECs induced a significantreduction in the density of neurons comparing with non-treatedco-cultured or treated with supernatants from non-infected cells(p