EVALUATION OF MITOCHONDRIAL DNA MASS IN WOMEN WITH POLYCYSTIC OVARY SYNDROME

VELAZQUEZ ME; MOTTA AB; ROJO M; PAUTASSO, MC; CERRONE GE; MILLÁN A; ABRUZZESE GA

Virtual

Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica.; 2020

SAIC, SAI, SAFIS

ObjectivePolycystic ovary syndrome (PCOS) is characterized by insulin resistance (RI) which can influence the content of mitochondrial DNA(mtDNA). Our objective was to evaluate the content of mtDNA inwomen with PCOS compared with control women and the relationwith metabolic parameters.Materials and methods:We studied fifty women with PCOS and thirty-four control womenaged 17 to 45 years. The determination of the number of copies ofmtDNA was carried out in peripheral blood leukocytes by Quantitative real-time PCR. The results were calculated using the comparative-cycle threshold (ΔΔCtq) method. Statistical analysis were carried out by Student?s t-test, correlation and linear regressions with asignificance level of 0.05 (SPSS 25).Results:Compared to controls, PCOS patients have higher weight, bodymass index (BMI), waist circumference (WC), also higher levels ofTG, LDL, total cholesterol, fasting plasma glucose and lower levelsof HDL cholesterol. By linear regression we observed that mtDNAwas significantly lower in the presence of PCOS (135.57 ± 81.34vs 190.37 ± 135.19; p = 0.023). A significant negative correlationwas observed between the mtDNA content and telomere length (p= 0.034). Within PCOS patients, women with insulin resistance according to a HOMA index ≥ 2.5 have a lower mtDNA content compared to PCOS women with HOMA