Differentiation of monocytes into macrophages in the presence of B. abortus generates a different phenotype.

A PESCE VIGLIETTI, J RODRÍGUEZ, MV DELPINO, GH GIAMBARTOLOMEI Y AM RODRÍGUEZ.; ANA JULIA BAZAN BOUYRIE

Congreso; Reunion Anual de Sociedades de Biociencias; 2020

Brucellosis is an infectious disease caused by bacteria of the genus Brucella that triggers a complex host response involving both innate and adaptive immunity. Monocytes and macrophages are not only the first line of defence against Brucella infection but also a main reservoir for the bacteria. Monocytes are recruited in large numbers to sites where the inflammatory process exists and the microenvironment will condition their differentiation to distinct functional profiles of macrophages and dendritic cells. How Brucella could modulate this process remains unclear. The aim of this study was to characterize the phenotype and functions of monocyte-derived macrophages differentiated in the presence of B. abortus. For this, human purified monocytes were cultured with MCSF in presence or absence (control) of heat-killed B. abortus (HKBa) for 5 days. Expression of CD40, HLADR and CD54 was quantified by flow cytometry. Cells showed a lower expression of CD54 and HLADR on monocyte-derived macrophages differentiated in the presence of HKBa than in control (p