ROLE OF Staphylococcus aureus CAPSULAR POLYSACCHARIDE IN OSTEOCLASTOGENESIS

ANA GEHRKE; DELPINO M. VICTORIA; SULIGOY CM; MARISA I. GOMEZ; DANIEL O. SORDELLI

San Miguel de Tucumán

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología (SAI)

Staphylococcus aureus isolates lacking capsular polysaccharide (CP) expression (NT) are frequently recovered from patients with chronic osteomyelitis. It has been proposed that the loss of CP expression during long term bone infection is the consequence of bacterial microevolution in the bone and it could represent an adaptive advantage contributing to bacterial persistence. Considering that the presence of S. aureus in the bone microenvironment enhances osteoclastogenesis and that loss of CP is associated to chronic osteomyelitis, the aim of this study was to determine the impact of CP expression in osteoclast differentiation. We determined the ability of different S. aureus strain pairs-encapsulated (CP5+) and nonencapsulated (NT) - to induce osteoclast differentiation using RAW 264.7 cells as precursors. The S. aureus Reynolds strain CP5+ induced significantly decreased osteoclastogenesis than its genetically obtained NT derivative (p