ANP enhances antioxidant capacity in experimental acute pancreatitis

ANA PAULA COURREGES; GUADALLUPE ALVAREZ; FABIANA LAIRON; MARISA REPETO; LILIANA G. BIANCIOTTI; MARIO CONTIN; MARCELO S. VATTA

Mar del Plata

Congreso; S A I C . S A F E . S A B . SAP 2 0 1 9; 2019

Sociedad Argentina de Investigación Clínica

In previous studies, we showed that ANP attenuates the severity of experimental AP by reducing trypsinogen activation and the inflammatory response. Recent studies support that endoplasmic reticulum (ER) stress and oxidative stress (OS) precedes these events. Indeed, we showed that ANP attenuates ER stress and favors ER dependent apoptosis. ER stress has been linked to OS in the pathophysiology of numerous diseases. OS is the imbalance between oxidant species and antioxidant defences. The pancreatic tissue is more susceptible to oxidative stress than other tissues because of extremely weak expression of antioxidative enzymes. Therefore in this work we sought to establish whether ANP affected OS in experimental AP by studding the main antioxidant (enzymatic and non enzymatic) defense. AP was induced in Sprague-Dawley strain rats (200-220g) by four repetitive cerulein injections (40μg/Kg). Thirty minutes before the first cerulein injection animals were infused with either saline (control) or ANP (1μg/Kg/h) for 60 min. Following euthanasia (60 min after the last cerulein injection) pancreatic samples were harvested for immunohistochemistry and enzymatic assays. ANOVA followed by a Student?s t test modified by Bonferroni was used for statistical analysis. Results are expressed as the means±S.E.M. and p values of 0.05 or less were considered statistically significant. ANP showed a translocation to nucleus of Nrf-2 (p