INIGEM   23989
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Human CD8+HLA-DR+ regulatory T cells share phenotypic and functional features with classical CD4+Foxp3+ regulatory T cells
Autor/es:
BAZ PLÁCIDA; MACHICOTE ANDRÉS; FAINBOIM LEONARDO; BELÉN SANTIAGO
Lugar:
Amsterdam
Reunión:
Congreso; 5th EUROPEAN CONGRESS of IMMUNOLOGY; 2018
Resumen:
We previously described a human CD8+ regulatory T cell subset, constitutively expressing the HLA-DR molecule (CD8+HLA-DR+ Tregs), which suppresses theproliferation of autologous PBMCs through cellular contact and CTLA-4. We aimed to characterize the regulatory signature of these Tregs, to identify additional mediators involvedin the regulatory mechanism, and to evaluate their exhaustion status.Materials and Methods: Peripheral and cord blood samples were obtained from healthy donors. Phenotypic markers were analyzed by flow cytometry. Suppression assays wereperformed with CellTrace Violet-stained PBMCs as responders, co-cultured with sorted CD8+HLA-DR+ or CD8+HLA-DR- cells, and activated with aCD3/aCD28. Cytokine secretion wasanalyzed after PMA/Ionomycin stimulation.Results: In comparison with CD8+HLA-DR- cells, CD8+HLA-DR+ Tregs showed an increased frequency of PD-1 (p