Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection.

ALDANA TROTTA; LUCIANA BALBOA, ; PAULA BARRIONUEVO; MARIA AYELEN MILILLO; M. VICTORIA DELPINO; ANA MARIA RODRIGUEZ; GUILLERMO GIAMBARTOLOMEI

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología (SAI). 14-17 Noviembre 2018.; 2018

Brucellosis is an infectious disease elicited by bacteria of the genusBrucella. Platelets have recently got involved in the modulationof innate and adaptive immune responses. We have previously reportedthat platelets act as carriers of bacteria, promoting the invasionof monocytes. Thus, the aim of this study was to further investigatethe role of platelets in the immune response against Brucella.First, we wondered whether the presence of platelets modulates thetime course of B. abortus infection. For this, THP-1 cells (humanmonocytic cell line) were infected with B. abortus in presence orabsence of platelets. Then, extracellular bacteria were killed andcells were incubated for different times. Our results demonstratethat the presence of platelets significantly increased the percentageof B. abortus-infected THP-1 cells at early time-points (p