INIGEM   23989
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Brucella abortus-STIMULATED PLATELETS ACTIVATE BRAIN MICROVASCULAR ENDOTHELIAL CELLS
Autor/es:
ALDANA TROTTA ; PAULA BARRIONUEVO ; ANA MARIA RODRIGUEZ ; MARÍA VICTORIA DELPINO; MARÍA CRUZ MIRAGLIA ; GUILLERMO HERNAN GIAMBARTOLOMEI
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Biociencias 2017.; 2017
Resumen:
Central nervous system invasion by bacteria of the genus Brucella results in an inflammatory disorder called neurobrucellosis. Bloodbrain barrier activation is a common feature of human neurobrucellosis, but the underlying mechanisms are largely unknown. The aim of this work was to investigate the influence of B. abortus (B.a.)-activated platelets (PTL) on human brain microvascular endothelial cells (HBMEC). For this, supernatants (SN) from B.a.-stimulatedhuman PTL were used to stimulate HBMEC. Expression of ICAM-1(CD54) and secretion of IL-6, IL-8 and MCP-1 were evaluated todetermine HBMEC´ activation. SN from B.a.-stimulated PLT induceda significant secretion of IL-6, IL-8, MCP-1 and the up-regulation ofCD54 expression in a dose-dependent manner compared with SNfrom unstimulated PLT (p0.05), demonstrating that a solublefactor released from PLT was implicated on HBMEC? activation. SNfrom heat-killed B.a.-stimulated PTL also activated HBMEC indicatingthat B.a.? viability was not necessary in activating PLT. SolubleCD40L was not implicated on HBMEC? activation, as an anti-CD40Lantibody could not block cytokine secretion or ICAM-1 up-regulation.Treatment of SN with trypsin eliminate the effect (p