INIGEM   23989
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Nonalcoholic fatty liver disease: Resistin differentially regulates T cell activation and reactive oxygen species levels
Autor/es:
GARCIA DANIEL; COLOMBATO LUIS; CECILIA GARCIA; A. C. CHERÑAVSKY; BENAVIDEZ JAVIER; ALEGRE NADIA S.; PONCINO DANIEL; BAZ PLACIDA
Lugar:
Roma
Reunión:
Workshop; Target-oriented apprioach to diagnosis and pharmacotherapy of NASH: a dialoque beween academia and industria; 2017
Institución organizadora:
Eur Association for the study of liver diseases
Resumen:
Human resistin (hRES) is a cytokine which plasma concentration and hepatic overexpression have been found elevated in Nonalcoholic Fatty Liver Disease (NAFLD) patients. Although hRES is produced by immunological cells, its effects on them are poorly understood. It has been proposed that hRES receptor is adenylyl cyclase-associated protein 1 (CAP1), which expression was only reported in THP-1 monocyte cell line. We aimed to evaluate hRES-mediated modulation of T cell early (CD69) and late (CD25) activation markers, hRES ability to modulate reactive oxygen species (ROS) production and CAP1 expression in peripheral blood mononuclear cells (PBMC) from NAFLD patients and controls (Co). PBMC were obtained from NAFLD patients (n= 23) and Co (n= 35) and evaluated for CAP1 expression. Isolated T cells were activated with coated anti-CD3 (3 ug/ml) +/- hRES (10 ng/ml) for 24 or 72 h and studied for CD69 or CD25 expression. In order to evaluate ROS levels, PBMC were incubated with or without hRES (20 ng/ml) for 24 h and stained with 2´7´-dichlorofluorescin diacetate (DCFH-DA). As a preliminary approach, monocyte oxidative burst was stimulated with phorbol 12-myristate 13-acetate (PMA, 100 ng/ml) +/- hRES and ROS production was evaluated using DCFH-DA. Cells were stained with anti-CD3, -CD4, -CD8 or -CD14 antibodies for T cells or monocytes identification. Protein expression and ROS production were evaluated by flow cytometry. Mann-Whitney and Wilcoxon paired test were used. Compared with Co, NAFLD patients showed increased CAP1 expression in monocytes (p