Fever-Range hyperthermia improves the anti-apoptotic effect induced by low pH on human neutrophils promoting a proangiogenic profile. Fernando

CABRERA MAIA; MARIA VICTORIA DELPINO; JORGE GEFFNER; EZEQUIEL DANTAS; CONSTANZA ARRIOLA BENITEZ; ANALÍA TREVANI; FERNANDO ERRA DIAZ; JOSEFINA CASTRO MAZA; NORBERTO SAN JUAN

Mar del Plata

Congreso; 61. LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI). 15-19 Noviembre 2016.; 2016

Neutrophils have the shortest lifespan among leukocytesand usually die via apoptosis. We previously reportedthat low pH, a hallmark of inflammatory processesand solid tumors, moderately delays neutrophil apoptosis.Here we analyzed whether fever-range hyperthermia(39.5°C) was able to modulate the anti-apoptotic effectinduced by low pH (pH 6.0). Hyperthermia markedly increasedneutrophil survival induced by low pH (anexinV/PI staining/flow cytometry): % apoptosis = 72±5, 45±6,70±7, and 19±3, for neutrophils cultured for 18 h at pH7.3/37ºC, pH 6.0/37°C, pH 7.3/39.5ºC, and pH 6.0/39.5°C,respectively (p