INIGEM   23989
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
B. abortus modulates osteoblast function through the induction of autophagy.
Autor/es:
AYELÉN IVANA PESCE VIGLIETTI,; MARÍA VICTORIA DELPINO; GUILLERMO HERNÁN GIAMBARTOLOMEI; PAULA CONSTANZA ARRIOLA BENITEZ
Reunión:
Congreso; 60. LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI). 15-19 Noviembre 2016.; 2016
Resumen:
Osteoarticular brucellosis is the most commonlocalization of human active disease. Osteoblasts are specializedmesenchyme-derived cells involved in bone formation and are considered asprofessional mineralizing cells. We demonstrated that B. abortus infection modified osteoblast metabolism by theinhibition of the deposition of organic and mineral matrix, leading to boneloss. B. abortus replicative vacuoleinvolved autophagy pathway activation and on the other hand, autophagy has beeninvolved in osteoblast metabolism. Then experiments were conducted to determineif B. abortus modulates osteoblastfunction through the activation of autophagy. To this end, B.abortus infected osteoblasts cells were lysed to determine LC3II, Beclin-1and p62 expression by Western blot. MMPsproduction was determined by gelatin zymography, collagen deposition by Siriusred staining and alizarin red Sstaining to determine calcium deposition. B. abortus infection increased the levels of LC3II and Beclin-1 andinhibited p62 expression indicating autophagy pathway induction. In additionwhen B. abortus infection experimentswere performed in the presence of bafilomycin A or chloroquine we did not observeinhibition of deposition of mineral and organic matrix. Taking together our resultsindicated that B. abortus induced theactivation of autophagy pathway in osteoblast cells and this activation isinvolved in the modulation of osteoblast function and bone formation.