Curcumin has prophylactic effects on effector memory T cell subset in a murine model of Nonalcoholic steatohepatitis

CECILIA GARCIA; INZAUGARAT, ME; ALEGRE NADIA S.; BAZ PLACIDA; E DE MATTEO; WALD M; A. C. CHERÑAVSKY

CABA

Congreso; 1st LASID-SAI-FAIC Meeting; 2015

SAI-LASID-FAIC

Background: Curcumin (Cur) is an antioxidant/antinflammatory polyphenol able to prevent metabolic alterations of experimental nonalcoholic fatty liver disease (NAFLD). We previously described an altered blood/liver pattern of CD4 / CD8 central and effector memory T cells (TCM, TEM) in NAFLD patients. We aimed to evaluate HFD-induced alterations in memory and naive T cells and Cur´s ability to prevent these potential alterations. Methods: C57BL/6 male mice were fed normal chow (NC) or HFD (10 mice/group) for 23 weeks. After 4 weeks, Cur (2g/kg diet) was co-administered to half mice of each group. After treatment, an intraperitoneal glucose tolerance test was performed and serum cholesterol was assessed. Liver tissue was processed for histological NAFLD Activity scoring . CD4 and CD8 cell subsets of TEM (CD44highCD62L-), TCM (CD44highCD62L+) and naive (CD44lowCD62L+) were analyzed by flow cytometry in liver, spleen and lymph nodes. Two-way ANOVA with Bonferroni´s post-test was used.Results: Cur improved increased liver injury, cholesterol levels and glucose intolerance (p