Prolonged inhibition of tumoral progression by in vivo administration of RNA silencing for AKT1

HERRERA PAEZ JJ, ALIPPE Y, COLOMBO L, VANZULLI S, BIANCOLINI C, CARRERAS MC, PODEROSO JJ.

Bariloche

Congreso; The Second South American Spring Symposium in Signal Transduction and Molecular Medicine. SISTAM 2012; 2012

The serine/threonin kinase Akt1 regulates apoptotic activity, cell proliferation and cell metabolism, and it is one of the most frequently activated protein kinase in cancer. We reported earlier that Akt1 activity is redox-regulated, since subtle augments in oxidative state increases p- AKT1 levels in mitochondria and thus prevents its translocation to the nucleus. Our aim was to study the effects of inhibiting Akt1 in LP07 tumors by means of siRNA for Akt1 (siAkt1). Experiments were carried out on Balb/C mice of 60 days old, which were subcutaneously inoculated with LP07 lung tumor cells. Tumors between 30 and 60 mm3 were transfected by electroporation with 25 μg of negative control silencing (siNC) or siAkt1. Tumors transfected with siAkt showed the highest regression at 48 hours (0.38±0.04 vs. 0.18±0.01 mm3 p