INIGEM   23989
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Mitochondrial nitric oxide modulates Sirt 3 activity delaying age-related mitochondrial damage
Autor/es:
PEREZ H, ALIPPE Y, CONVERSO DP, ELGUERO ME, PODEROSO JJ, CARRERAS MC
Lugar:
San Carlos de Bariloche
Reunión:
Congreso; Second South American Spring Symposium in Signal Transductionnand Molecular Medicine; 2012
Resumen:
D. Harman proposed the oxidative stress theory of aging. We explored redox behavior of liver and brain mitochondria during aging in 3-24 mo. male/female rats. By Western blots and RT-PCR we observed increased nNOS transcription, translation and translocation with a significant progressive increment in mtNOS levels and NO production measured with DAF-FM by flow citometry. Wb against 3-nitrotyrosine antibody evidenced nitration of mitochondrial proteins at 18 mo. and 24 mo. and by imnunoprecipitation, we confirmed the nitration of Complex I, MnSOD and Sirt3. The progressive reduction in MnSOD activity (3 mo=1394±95 Vs 24 mo=921±11 nmoles/min.mg; p