INIGEM   23989
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Unidad Ejecutora - UE
artículos
Título:
Metagenomic analysis of gut microbiota in non-treated plaque psoriasis patients stratified by disease severity. Development of a new psoriasis-microbiome index
Autor/es:
LEONELA LUCE; IGNACIO DEI CAS; HERNÁN DOPAZO; FLORENCIA GILIBERTO; ALBERTO PENAS‑STEINHARDT; LEONELA LUCE; IGNACIO DEI CAS; HERNÁN DOPAZO; FLORENCIA GILIBERTO; ALBERTO PENAS‑STEINHARDT
Revista:
Scientific reports
Editorial:
Nature
Referencias:
Año: 2020
ISSN:
2045-2322
Resumen:
Psoriasis is an immune-mediated skin disorder. Imbalance of gut microbial populations has beenimplicated in many diseases. We aimed to investigate whether there were differences in gutmicrobiota in psoriasis patients vs non-psoriasis controls and between psoriasis severity groups. 55psoriasis patients and 27 controls were included. V3?V4 regions of the 16S rRNA gene of fecal sampleswere analyzed using Illumina MiSeq. Bioinformatic analysis was performed. We found changesin gut microbiome composition depending on their psoriasis status as determined by weightedunifrac (p < 0.05), in particular an increase in Firmicutes and depletion of Bacteroidetes in psoriasispatients. Additionally, the Faecalibacterium and Blautia genus were higher in psoriasis patients whileBacteroides and Paraprevotella in non-psoriasis controls (p < 0.05, LDA score > 2). Moderate-to-severepsoriasis patients had lower biodiversity than mild psoriatic patients (p = 0.049). No differences forbeta-diversity were found. We developed a Psoriasis-Microbiota Index (PMI), which discriminatedamong psoriasis patients and controls with sensitivity: 0.78 and specificity: 0.79. Furthermore, weperformed a meta-analysis with published data to validate this index. We demonstrated gut dysbiosisin psoriasis patients, suggesting a role in psoriasis pathophysiology. Furthermore, we developeda PMI with the potential to discriminate between psoriasis patients and controls across differentpopulations, which could be used as a biomarker in the clinical practice.