INIGEM   23989
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Unidad Ejecutora - UE
artículos
Título:
Endocrine modulation of Brucella abortus-infected osteocytes function and osteoclastogenesis via modulation of RANKL/OPG
Autor/es:
AYELÉN IVANA PESCE VIGLIETTI; AYELÉN IVANA PESCE VIGLIETTI; MARIA VICTORIA DELPINO; MARIA VICTORIA DELPINO; GUILLERMO HERNÁN GIAMBARTOLOMEI; GUILLERMO HERNÁN GIAMBARTOLOMEI
Revista:
MICROBES AND INFECTION
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2019
ISSN:
1286-4579
Resumen:
Osteoarticular brucellosis is the most frequentcomplication of active disease.A large amount of cells in bone are osteocytes. Since bone remodeling process is regulated by hormones we sought tostudy the effect of cortisol and DHEA in B.abortus-infectedosteocytes. Cortisol treatment inhibited the expression of IL-6, TNF-α, MMP-2 andRANKL in B. abortus-infected osteocytes.DHEA could reverse theinhibitory effect of cortisol on MMP-2 production. B. abortus infection inhibited connexin 43 (Cx43) expression inosteocytes. This expression wasincreased when cortisol was incorporated during the infection and DHEAtreatment partially reversed the effect of cortisol. Osteocytes-infected with B.abortus induced osteoclast´s differentiation. Yet, the presence ofcortisol, but not DHEA, during osteocyte infection inhibited osteoclastogenesis.Glucocorticoid receptor (GR) is implicated in the signaling of cortisol. Infectionwith B. abortus was able to increase GRα/βratio. Levels of intracellular cortisolare not only dependent on GR expression but also a result of the activity ofthe isoenzymes 11β-hydroxysteroiddehydrogenase (11β-HSD)-1 (cortisone to cortisol conversion), 11β-HSD2 (cortisol to cortisone conversion). B. abortus infection increased 11β-HSD1/ 2 ratio and cortisone mimicked the effect of cortisol. Our results indicatedthat cortisol and DHEA could modulate osteocyte responses during B. abortus infection.