Salivary bone turnover markers in healthy pre- and postmenopausal women: daily and seasonal rhythm

PELLEGRINI G; GONZALES CHAVES M; FAJARDO M; PONCE G; TOYOS G; LIFSHITZ F; FRIEDMAN S; ZENI S

CLINICAL ORAL INVESTIGATIONS.

SPRINGER HEIDELBERG

Año: 2012 vol. 16 p. 651 - 657

1432-6981

No studies had investigated circadian and circan-No studies had investigated circadian and circan- 13 nual rhythms of bone biomarkers in whole saliva. Wenual rhythms of bone biomarkers in whole saliva. We 14 evaluated the salivary daily and seasonal rhythm ofevaluated the salivary daily and seasonal rhythm of 15 carboxy-terminal telopeptide of type I collagen (CTX) andcarboxy-terminal telopeptide of type I collagen (CTX) and 16 bone alkaline phosphatase (b-ALP). Forty clinical and oralbone alkaline phosphatase (b-ALP). Forty clinical and oral 17 healthy ambulatory pre- and postmenopausal women fromhealthy ambulatory pre- and postmenopausal women from 18 two southern Argentine cities: Comodoro Rivadavia (lati-two southern Argentine cities: Comodoro Rivadavia (lati- 19 tude 45º S) and Ushuaia (latitude 54º S) were included intude 45º S) and Ushuaia (latitude 54º S) were included in 20 the study. CTX levels were evaluated in serum, urine, andthe study. CTX levels were evaluated in serum, urine, and 21 saliva, and b-ALP levels were measured in serum andsaliva, and b-ALP levels were measured in serum and 22 saliva. In both groups of women, salivary CTX showed asaliva. In both groups of women, salivary CTX showed a 23 maximum percentage of change early in the morning (80%)maximum percentage of change early in the morning (80%) 24 and a minimum in the late afternoon (45%), similarly to theand a minimum in the late afternoon (45%), similarly to the 25 pattern observed in urinary samples. No daily rhythm was observed in serum or salivary b-ALP. 25-Hydroxyvitamin pattern observed in urinary samples. No daily rhythm was observed in serum or salivary b-ALP. 25-Hydroxyvitamin D levels decreased in winter vs. summer (p<0.01) without p<0.01) without differences between the two studied groups. Conversely, parathormone reached higher levels in winter (p<0.05) p<0.05) which induced a slight non-significant increment in salivary CTX and b-ALP levels. The results showed that, as in serum and urinary samples, salivary CTX exhibits daily and a slight seasonal rhythmicity. Whole non-stimulated saliva is a useful tool to detect several oral and systemic diseases because it has important advantages compared to serum and urinary samples. Then, it may also be a promising sample to test changes in bone metabolism contributing to diagnose and to monitor the therapy of several metabolic bone diseases.