PPAR gamma and PPAR alpha expression and its relationship with immune -endocrine mediators during pulmonary tuberculosis treatment

DÍAZ A; DATTILIO, L; PENAS, F; CEVEY, A; BÉRTOLA, D; BOTTASO, O; GOREN, NB; BAY, M

Buenos Aires

Congreso; LXIII Argentinean Society for Immunology Meeting, II French-Argentinean Immunology meeting; 2015

Sociedad Argentina de Inmunología

Tuberculosis (TB) is a major health problem that requires an appropriate cell immune response (IR) to be controlled, although an exacerbated IR is also involved in tissue damage. We have found that TB patients at the time of diagnosis (T0) had elevated plasma levels of proinflammatory mediators, TGF -β and cortisol together with diminished concentrations of Dehydroepiandrosterone sulfate (DHEA-S) and decreased body mass index (BMI). These patients also showed an increased expression of peroxisome proliferator-activated receptors (PPAR) γ and α in their peripheral blood mononuclear cells (PBM). We investigated the expression of the 2 PPAR isoforms by real time PCR in PBM of pulmonary TB patients at T0 and at 2, 4 and 6 months of specific treatment. The cohort was composed of 39 adult patients with TB (dis ease severity: 9 mild, 15 moderate and 15 severe), 10 of them completing treatment and follow-up, and 20 age- and sex - matched healthy controls (HCo). Plasma cytokines were assessed by ELISA and Cortisol and DHEA -S by electrochemiluminescence. At T0 PPARγ t ranscripts were increased in patients respect HCo (p