ADAPTATION OF Staphylococcus aureus TO THE HOST DURING CHRONIC INFECTION IS ASSOCIATED WITH DECREASED VIRULENCE

SULIGOY MAURICIO; DOTTO CRISTIAN; LOMBARTE SERRAT ANDREA; RIVIERE AGUSTIN; SORDELLI DANIEL; BUZZOLA FERNANDA

Córdoba

Congreso; SAMIGE XI; 2015

Staphylococcus aureus is a highly prevalent opportunistic, multifactorial pathogen that can infect,replicate and persist in humans and domestic animals of economic importance. The S. aureusgenome carries a vast array of genes coding for virulence and evasion factors. The capacity of S.aureus to cause a wide variety of diseases partially depends on its ability to express a number ofthese factors that, acting in concert, permit adaptation of the pathogen to distinct and changingenvironmental niches during infection. The evolution of S. aureus within affected tissue appears toplay a key role in persistence of the microorganism and chronicity of the infection. Selection pressureexerted on S. aureus by host factors may determine the emergence of mutants better adapted to theevolving conditions at the infection site. There is undisputable evidence showing that certain diseasescaused by S. aureus, such as osteomyelitis, start as acute and later develop into a chronic illness.This study identified changes that occur in S. aureus exposed to the host defense mechanisms duringinfection and evaluated whether these changes affect the virulence of the organism. To this purpose,we studied S. aureus isolates from single patient with chronic osteomielitis and from the sameinfection site. In one case, strains SaT0 and SaT13 which were isolated 13-months apart, belonged inthe same clonal complex group (CC1) and shared the same sequence type (ST188), PFGE type, spatype (t189) and Agr type (type I). Strain SaT0 expressed capsular polysaccharide type 8 (CP8) (at thebeginning of the infectious process) whereas strain SaT13 did not (13 months later). Furthermore, thestrain SaT0 expressed alpha and beta- haemolysins whereas strain SaT13 did not. It wasdemonstrated by real time PCR that these phenotypic differences were due to the lack of expressionin strain SaT13 of the effector molecule of the global regulator agr (RNAIII). S. aureus strains weretested in a rat model of osteomyelitis and the bacterial load (CFU/tibia) and the morphometricosteomyelitic index (OI) were determined. The bacterial load and the OI of the agr deficient strain,which were unable to produce CP, were significantly lower than those of the parental wild-type. Nosignificant differences were found in the bacterial load or the OI from rats challenged with the isogenicReynolds strains (CP5, CP8 and NT non-typeable) indicating that lack of capsule expression alonewas not the sole responsible for reduced virulence of the mutants. Small colony variants (SCVs)emerged in the tibias of rats experimentally infected with all S. aureus strains tested and the number ofSCV CFUs increased with the length of the infection process. Whereas there is no doubt that lack ofagr-dependent factors turns S. aureus less virulent, mutations that alter the agr functionality seem topermit a better adaptation of S. aureus to the host for persistence and infection chronicity.