IMPAM   23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Staphylococcus aureus Sbi and SpA proteins have important effects in the development of sub-cutaneous abscess in mice
Autor/es:
GONZALEZ CINTIA D; LEDO CAMILA; GOMEZ MARISA I.
Lugar:
PISA
Reunión:
Seminario; GORDON RESEARCH SEMINAR STAPHYLOCOCAL DISEASES; 2015
Institución organizadora:
GORDON RESEARCH CONFERENCES
Resumen:
Staphylococcus aureus is an important human pathogen that is the main causative agent of skin and soft tissue infections. The protein A (SpA) and the Staphylococcal binding immunoglobulin protein (Sbi) are strong evasion factors. In addition, we have demonstrated their ability to induce the production of pro-inflammatory cytokines and chemokines in vitro and in vivo. This study was aimed at elucidating the contribution of SpA and Sbi to the development of abscess in a model of skin infection, in which an appropriate inflammatory response is known to be required to contain the bacteria. Mice were inoculated by subcutaneous route with 1x108 CFU of S.aureus strain Newman or the isogenic mutants that do not express Sbi (Sbi-), SpA (SpA-) or both (SpA-Sbi-). S.aureus caused abscesses with a median area of 0.698 cm2 (min. 0.35; max 1.09), and a bacterial burden of 3.52x107 CFU (min. 8x106; max. 2.37x108) at day three after inoculation. Bacterial dissemination to the lung, spleen, liver and kidney was observed in more than 64% percent of the animals. The bacterial burden in the abscesses induced by the Sbi- mutant was significantly higher than that observed in S.aureus inoculated mice (p less than 0.05). The areas of the abscesses induced by SpA- and the SpA-Sbi- mutant were significantly bigger than those evoked by S.aureus (p less than 0.001 and p