A Brucella spp. protease inhibitor is a useful adjuvant in oral vaccine formulations against infectious diseases.

CARABAJAL MARIANELA; ANDRES IBAÑEZ; GS RISSO; CORIA LM; GONZALES COBIELLO PL; BRUNO, LAURA; FRANK FERNANDA; BRIONES GC; KARINA PASQUEVICH; CASSATARO JULIANA

Berlin

Congreso; Brucellosis 2014 International Research Conference; 2014

Introduction: Previous results showed that a Brucella spp. protein (BP) administered by the oral route and without adjuvants induced protection against oral Brucella challenge. BP is a broad spectrum protease inhibitor with a stronger activity against serine proteases secreted by the pancreas to the intestine (elastase, trypsine and alpha chymotrypsin).These previous results prompted us to study its potential application as oral adjuvant for other antigens (Ags). Methods and Results: As BP is able to inhibit in vitro the activity of many proteases present at the gastrointestinal tract we directed all our efforts to study its potential application in oral vaccine delivery. We showed that in BALB/c or C57BL/6 mice (n=6) oral delivery of OVA in the presence of BP resulted in a significantly increased OVA-specific T cell response (DTH assay; P