IMPAM   23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
SHEWANELLA SPECIES AS POTENTIAL HORIZONTAL TRANSFER VECTORS OF S.MA.I2-LIKE GROUP II INTRONS
Autor/es:
CECILIA QUIROGA; SUSANA C. VAZQUEZ; GISELA PARMECIANO DI NOTO; WALTER P MAC CORMACK; DANIELA CENTRON
Lugar:
Rosario
Reunión:
Congreso; IX CONGRESO ARGENTINO DE MICROBIOLOGÍA GENERAL; 2013
Resumen:
Bacterial group II introns are widely distributed ribozymes capable of self-splicing. These elements have the ability to move to new regions in a genome through an RNA intermediate by a retrotransposition event. Previously, we found that a class C- group II intron S.ma.I2 from the clinical isolate Serratia marcescens shares a common ancestor with group II introns encoded in marine bacteria. Interest in this genus arose from its biotechnological application as biofuel cells and bioremediation, and recently also due to its relevance as an opportunistic pathogen in soft tissue lesions. The aim of this work was to evaluate the occurrence of S.ma.I2-like class C group II introns among Shewanella isolates and to characterize their genomic contexts. We searched for S.ma.I2 homologues in 10 clinical and 5 marine Shewanella spp. isolates by PCR using specific primers. Three out of 15 isolates harboured a class C group II intron, corresponding to 1 clinical (S. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingS.ma.I2 from the clinical isolate Serratia marcescens shares a common ancestor with group II introns encoded in marine bacteria. Interest in this genus arose from its biotechnological application as biofuel cells and bioremediation, and recently also due to its relevance as an opportunistic pathogen in soft tissue lesions. The aim of this work was to evaluate the occurrence of S.ma.I2-like class C group II introns among Shewanella isolates and to characterize their genomic contexts. We searched for S.ma.I2 homologues in 10 clinical and 5 marine Shewanella spp. isolates by PCR using specific primers. Three out of 15 isolates harboured a class C group II intron, corresponding to 1 clinical (S. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingSerratia marcescens shares a common ancestor with group II introns encoded in marine bacteria. Interest in this genus arose from its biotechnological application as biofuel cells and bioremediation, and recently also due to its relevance as an opportunistic pathogen in soft tissue lesions. The aim of this work was to evaluate the occurrence of S.ma.I2-like class C group II introns among Shewanella isolates and to characterize their genomic contexts. We searched for S.ma.I2 homologues in 10 clinical and 5 marine Shewanella spp. isolates by PCR using specific primers. Three out of 15 isolates harboured a class C group II intron, corresponding to 1 clinical (S. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearing. Interest in this genus arose from its biotechnological application as biofuel cells and bioremediation, and recently also due to its relevance as an opportunistic pathogen in soft tissue lesions. The aim of this work was to evaluate the occurrence of S.ma.I2-like class C group II introns among Shewanella isolates and to characterize their genomic contexts. We searched for S.ma.I2 homologues in 10 clinical and 5 marine Shewanella spp. isolates by PCR using specific primers. Three out of 15 isolates harboured a class C group II intron, corresponding to 1 clinical (S. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingS.ma.I2-like class C group II introns among Shewanella isolates and to characterize their genomic contexts. We searched for S.ma.I2 homologues in 10 clinical and 5 marine Shewanella spp. isolates by PCR using specific primers. Three out of 15 isolates harboured a class C group II intron, corresponding to 1 clinical (S. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingShewanella isolates and to characterize their genomic contexts. We searched for S.ma.I2 homologues in 10 clinical and 5 marine Shewanella spp. isolates by PCR using specific primers. Three out of 15 isolates harboured a class C group II intron, corresponding to 1 clinical (S. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingS.ma.I2 homologues in 10 clinical and 5 marine Shewanella spp. isolates by PCR using specific primers. Three out of 15 isolates harboured a class C group II intron, corresponding to 1 clinical (S. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingS. algae) and 2 marine isolates (Shewanella spp. and S. vesiculosa) isolates. Nucleotide sequence analysis showed that these introns were highly identical to S.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingS.ma.I2 (> 95%). Comparative analysis of Shewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearingShewanella genomes using Artemis showed that out of 24 complete projects, 5 harboured at least one class C group II intron in their chromosomes. In addition, class C group II introns were found in the boundaries of different transposases. We looked for these genes in our 3 intron-bearing Shewanella isolates by PCR; however none of these S.ma.I2-like group II introns were associated to them. In order to identify their insertion sites we did inverse PCR using specific primers. Our results showed that they were inserted in the intergenic region of unknown proteins with no clear association to mobile elements. Sequence analysis of the insertion site using the MFOLD software confirms that alike S.ma.I2, our S.ma.I2-like group II introns also insert downstream of DNA secondary structures. Our experimental and in-silico analyses support the hypothesis that the genus Shewanella may act as a vector that promotes the transfer of S.ma.I2-like group II introns from a marine niche to a clinical environment.isolates by PCR; however none of these S.ma.I2-like group II introns were associated to them. In order to identify their insertion sites we did inverse PCR using specific primers. Our results showed that they were inserted in the intergenic region of unknown proteins with no clear association to mobile elements. Sequence analysis of the insertion site using the MFOLD software confirms that alike S.ma.I2, our S.ma.I2-like group II introns also insert downstream of DNA secondary structures. Our experimental and in-silico analyses support the hypothesis that the genus Shewanella may act as a vector that promotes the transfer of S.ma.I2-like group II introns from a marine niche to a clinical environment.S.ma.I2, our S.ma.I2-like group II introns also insert downstream of DNA secondary structures. Our experimental and in-silico analyses support the hypothesis that the genus Shewanella may act as a vector that promotes the transfer of S.ma.I2-like group II introns from a marine niche to a clinical environment.Shewanella may act as a vector that promotes the transfer of S.ma.I2-like group II introns from a marine niche to a clinical environment.S.ma.I2-like group II introns from a marine niche to a clinical environment. IX