IMPAM   23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Acinetobacter baumannii clinical isolates motility and biofilm formation
Autor/es:
MARÍA SILVINA STIETZ; MARÍA SOLEDAD RAMÍEZ; DANIELA CENTRÓN; MARIANA CATALANO
Lugar:
Cologne
Reunión:
Simposio; 9th International Symposium on the Biology of Acinetobacter; 2013
Institución organizadora:
University of Cologne
Resumen:
Objective: Characterisation of clinical isolates from Clonal Complex (CC) and PFGE types prevalent in Buenos Aires hospitals by motility and biofilm formation.. Methods: Sixteen clinical isolates and A. baumannii ATCC19606, ATCC17978 and A118 (control strains) were included. Static biofilm assays were performed using microtiter plate incubated at 25º and 37ºC for 24 h and stained with crystal violet (0.5% w/v). Motility was tested in Luria-Bertani medium containing 0.4% of agar and 0.05% of triphenyltetrazolium. The presence/absence of csuAB, csuA, csuB, csuC, csuD, csuE, pilU, pilY, pilA, fimA, pgaA, pgaB, pgaC, pgaD, ompA and bap genes were determined by PCR. Results: Six out of 14 clinical isolates (CC113B/CC79P, n: 5; CC103B/15P, n: 2; CC109B/1P, n: 3; CC110B/25P, n: 4) showed greater capability of biofilm formation than ATCC17978 (no producer) at 37°C and/or 25°C and were classified as higher and lower producers without correlation with CCs or PFGE types. The remaining two isolates (CC119B/CC79P and ST211B/ST176P) were higher and lower biofilm producers, respectively. All isolates showed twitching motility but varying in agar surface spread. Among the studied genes, isolates differed in the presence/absence of pilA and pilY, only. Conclusion: Our preliminary results showed that motility and the presence of pilis enconding genes did not correlated with biofilm formation. Also, biofilm formation seems not to be a particular feature of isolates from a single CC.