IMPAM   23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
microRNA expression profile in the metacestode stage of the human parasite Echinococcus granulosus.
Autor/es:
MACCHIAROLI N; KAMENETZKY L; CUCHER M; ROSENZVIT M C
Lugar:
Rosario, Argentina
Reunión:
Congreso; 4to. Congreso Argentino de Bioinformática y Biología Computacional (4CAB2C); 2013
Resumen:
microRNA expression profile in the metacestode stage of the human parasite Echinococcus
granulosus
Natalia Macchiaroli1, Laura Kamenetzky1, Marcela Cucher1, Mara Rosenzvit1
1IMPaM, UBA-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Argentina
microRNA (miRNAs) are a class of small (~22 nucleotides), non-coding RNAs that are key
regulators of gene expression at post transcriptional level and play essential roles in important
biological processes in animals, plants and viruses. Here we describe the microRNA expression
profile in the metacestode stage of an important human parasite, Echinococcus granulosus, using
Illumina deep sequencing combined with bioinformatic analysis and stem-loop reverse transcription
polymerase chain reaction. Deep sequencing of two small RNA libraries prepared from two
biological replicates of metacestode stage of E. granulosus produced 17.85 and 19.95 million raw
reads. After low quality reads filtering, adapter trimming and read collapsing, 633,445 and 599,373
unique sequences were retained from each library respectively. Comparative analysis of the
metacestode unique sequences to Echinococcus spp. miRNA sequences from protoscolex stage
deposited in the miRBase showed that almost the full miRNA repertoire was present, including
Echinococcus spp. specific miRNAs, with the exception of miR-7 and miR-1 suggesting that these
miRNAs could be specific of the protoscolex stage. Interestingly, miR-10 and let-7, two highly
conserved miRNAs across metazoans, were the most abundantly expressed; followed by miR-4989,
an Echinococcus spp. specific miRNA, and then by miR-71, a miRNA conserved only across
invertebrate species. Selected miRNAs were validated by stem-loop reverse transcription
polymerase chain reaction in two developmental stages of the parasite: metacestode and
protoscolex. Candidate novel miRNAs, additional conserved ones and other small RNA species are
under analysis with available tools for non-coding RNA discovery, profiling and functional
annotation based on high-throughput sequencing. This data will provide valuable information for
comparative analysis with the related species E. multilocularis, the causative agent of hydatid
disease in the north hemisphere.