IMPAM   23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Echinococcus granulosus: molecules involved in neurotransmission and development
Autor/es:
FEDERICO CAMICIA; MARCELA ALEJANDRA CUCHER; LAURA CECILIA PRADA; NATALIA MACCHIAROLI; LUCAS MALDONADO; LAURA KAMENETZKY; MARA C. ROSENZVIT
Lugar:
Florianopolis
Reunión:
Congreso; III Encontro de Parasitologia do Mercosul. 22-26 Octubre 2013. Florianópolis. Brasil.; 2013
Institución organizadora:
Qualidade Eventos-Eventos corporativos especiais
Resumen:
E. granulosus life
cycle involves definitive and intermediate hosts. The adult parasite develops
in the intestine of the definitive host and the hydatid cyst (metacestode),
that contains protoscoleces develops in the viscera of the intermediate host.
Due to safety reasons and material availability, most of our research was done
on the metacestode stage. However, it is important to obtain transcriptomic
data from other stages. E. granulosus
shows remarkable developmental plasticity .This feature plus the existence of
strains adapted to particular hosts makes E. granulosus an model for
development studies. We developed in
vitro cultures to be able to study parasite development. Starting with pig
strain protoscoleces, we observed differentiation towards strobilar stage
(pre-adult worms) by co-cultivation with rat hepatome cells and differentiation
towards vesicular direction obtaining pre-microcysts by co-culture with cos 7
cells. The models allowed to analyse the
ontogeny of the serotoninergic nervous system. We observed a higher level of
serotoninergic reactivity when the parasite develops to the strobilar
direction. We used the Worm Microtracker
(Simonetta and Golombeck, 2007) to analyse the effect of serotonin and inhibitors
of its transporters and receptors on parasite motility. This system would be
very useful for drug screening. We used
the in vitro, and also in vivo models, to study the regulation of expression by
microRNAs and showed for the first time that this mechanism exist in Echinoccus
spp. The models and systems developed or
adapted to cestodes in our lab will be very useful for providing material for
transcriptomics, proteomics and post genomic analyses in the frame of the
FlatDB.