INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs
PÉREZ, MATÍAS GASTÓN; LICHTENSTEIN, GABRIEL; MACCHIAROLI, NATALIA; CONTI, GABRIELA; ASURMENDI, SEBASTIÁN; MILONE, DIEGO HUMBERTO; STEGMAYER, GEORGINA; CUCHER, MARCELA; KAMENETZKY, LAURA; ROSENZVIT, MARA CECILIA; PÉREZ, MATÍAS GASTÓN; MACCHIAROLI, NATALIA; LICHTENSTEIN, GABRIEL; CONTI, GABRIELA; ASURMENDI, SEBASTIÁN; MILONE, DIEGO HUMBERTO; STEGMAYER, GEORGINA; KAMENETZKY, LAURA; CUCHER, MARCELA; ROSENZVIT, MARA CECILIA
INTERNATIONAL JOURNAL FOR PARASITOLOGY
ELSEVIER SCI LTD
Año: 2017 vol. 47 p. 643 - 643
MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia crassiceps, a laboratory model for T. solium studies, and identified miRNAs in the T. solium genome. Moreover, we analysed the effect of praziquantel, one of the two main drugs used for cysticercosis treatment, on the miRNA expression profile of T. crassiceps cysticerci. Using small RNA-seq and two independent algorithms for miRNA prediction, as well as northern blot validation, we found transcriptional evidence of 39 miRNA loci in T. crassiceps. Since miRNAs were mapped to the T. solium genome, these miRNAs are considered common to both parasites. The miRNA expression profile of T. crassiceps was biased to the same set of highly expressed miRNAs reported in other cestodes. We found a significant altered expression of miR-7b under praziquantel treatment. In addition, we searched for miRNAs predicted to target genes related to drug response. We performed a detailed target prediction for miR-7b and found genes related to drug action. We report an initial approach to study the effect of sub-lethal drug treatment on miRNA expression in a cestode parasite, which provides a platform for further studies of miRNA involvement in drug effects. The results of our work could be applied to drug development and provide basic knowledge of cysticercosis and other neglected helminth infections.