IMPAM 23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
artículos
Título:
Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
Autor/es:
CARNEVALE, S.; PANTANO, M.L.; KAMENETZKY, L.; MALANDRINI, J.B; SORIA, C. C; VELÁSQUEZ, J.N.
Revista:
ACTA PARASITOLOGICA
Editorial:
VERSITA
Referencias:
Lugar: Varsovia; Año: 2015 vol. 60 p. 211 - 217
ISSN:
1230-2821
Resumen:
Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for detecting specific F. hepatica infections in sheep.
