In vitro infection of human dura mater fibroblasts with Staphylococcus aureus colonization and reactive production of IL-1b

ALMIRÓN M.A.; GOLDSCHMIDT E.; MENDOZA BERTELLI A; GOMEZ M.I.; ARGIBAY P.; SANJUAN N.

NEUROLOGICAL RESEARCH.

MANEY PUBLISHING

Lugar: London; Año: 2015 vol. 37 p. 867 - 873

0161-6412

Objective: Post-operative meningitis, caused mainly by Staphylococcus aureus and Gram-negative rods,is a life-threatening complication after neurosurgery, and its pathogenesis is far from clear. The purpose ofthis work was to study the experimental infection of human dura-mater fibroblasts and whole human duraby S. aureus.Methods: In vitro cultures of human dura-mater fibroblasts and organotypic cultures of small pieces ofhuman dura mater were inoculated with a human-derived S. aureus strain. The pattern of bacterial infectionas well as cytokines secretion by the infected fibroblasts was studied.Results: Our results suggest that colonisation of human dura-mater fibroblasts in culture and whole duramatertissue by S. aureus includes bacterial growth on the cell surface, fibroblast intracellular invasion bybacteria and a significant synthesis of interleukin 1beta (IL-1beta) by the infected cells.Conclusion: This is the first report of human dura-mater fibroblast infection by S. aureus. Hopefully, theseresults can lead to a better understanding of the pathogenesis of meningitis caused by this bacterialspecies and to a more rational therapeutic approach.