IMPAM   23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
artículos
Título:
Effect of several PEO-PPO amphiphiles on bax, bcl-2 and hTERT mRNAs: an insight into apoptosis and cell immortalization induced in hepatoma cells by these polymeric excipients
Autor/es:
CASTRONUOVO CC; CUESTAS ML; OUBI√ĎA JR; MATHET VL
Revista:
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Editorial:
PORTLAND PRESS LTD
Referencias:
Lugar: Londres; Año: 2015
ISSN:
0885-4513
Resumen:
Recent data have shown that synthetic polymers and nanomaterials display phenotypic effects in cells and signal transduction mechanisms involved in inflammation, differentiation, proliferation, and apoptosis. AIM: This article aims to investigate the effect of poly(ethylene oxide)-poly(propylene oxide) (PEO-PPO) block copolymers with a wide range of biomedical and pharmaceutical applications on apoptosis and/or cell immortalization, by flow cytometry and multiplex RT-PCR for bax, bcl-2, and human telomerase reverse transcriptase (hTERT). RESULTS: PEO-PPO amphiphiles upregulated bax and hTERT and induced apoptosis of two human hepatoma cell lines. CONCLUSIONS: PEO-PPO block copolymers-considered safe for human use-can drastically alter gene expression profiles of genes related to apoptosis/cell proliferation.