INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
Aromatic compound-dependent Staphylococcus aureus is safe in a nasal colonization leukopenic murine model
BARBAGELATA, MS; ALVAREZ, LP; DOTTO, CM; LATTAR, SM; SORDELLI, DO; BUZZOLA, FR
International Journal of Microbiology
Lugar: New York; Año: 2012 p. 1 - 1
Staphylococcus aureus nasal carriage is a risk factor for individuals suffering trauma, surgical procedures, invasive devices and/or decreased immunity. Recently, we demonstrated that artificial nasal colonization with an attenuated S. aureus mutant reduced by bacterial interference the colonization of pathogenic strains of S. aureus. This could be an optional tool to diminish the rate of S. aureus infections in hospitalized patients. The aim of this study was to construct a safe ΔaroA mutant of S. aureus and to discriminate its from nasal colonizing and osteomyelitis S. aureus isolates by SmaI pulsed-field gel electrophoresis (PFGE) typing. The ΔaroA mutant, named RD17, exhibited an LD50 (3.2x106 colony-forming unit (CFU)) significantly higher than that of the parental strain (2.2x103 CFU). The colony number of RD17 mutant recovered from nares of leukopenic mice was similar to that observed in the animals of the control group. Therefore, the aroA mutant was demonstrated to be safe due to maintain low growth levels in the nares regardless of immune status of the animals. PFGE typing allowed the unequivocal identification of the S. aureus and differentiation of aroA mutants in nasal colonizing and osteomyelitis isolates. This information could be important to discriminate endogenous infections from laboratory strains of S. aureus.