INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
Novel glutamate dehydrogenase genes show increased transcript and protein abundances in mature tomato fruits
GISELA FERRARO; SANTIAGO BORTOLOTTI; PABLO MORTERA; ARMIN SCHLERETH; MARK STITT; FERNANDO CARRARI; LAURA KAMENETZKY; ESTELA M. VALLE
JOURNAL OF PLANT PHYSIOLOGY
Año: 2012 vol. 169 p. 899 - 899
NAD(P)H-glutamate dehydrogenase (GDH, EC 220.127.116.11) contributes to the control of glutamate homeo- stasis in all living organisms. In bacteria and animals, GDH is a homohexamer allosterically regulated, whereas in plants NADH-GDH (EC 18.104.22.168) is also found as heterohexamer of - and -subunits, but its regulation remains undeﬁned. In tomato (Solanum lycopersicum), GDH activity increases during the fruit ripening along with the content of free glutamate, the most abundant amino acid of ripe fruit involved in conferring the genuine tomato ﬂavour. In this work, novel Slgdh-NAD genes were identiﬁed in the recently deciphered tomato genome: three encoding the -subunit (Slgdh-NAD;A1-3) and one additional gene encoding the -subunit of GDH (Slgdh-NAD;B1) isolated from a genomic library. These genes are located in different chromosomes. Slgdh-NAD;A1-3 show conserved structures, whereas Slgdh-NAD;B1 includes a novel 5 -untranslated exon. Slgdh-NAD;A1-3 transcripts were detected in all tomato tissues examined, showing the highest levels in mature green fruits, contrasting with Slgdh-NAD;B1 transcripts which were detected mainly in roots or in mature fruits when treated with glutamate, NaCl or salicylic acid. Analyses of GDH activity and protein distribution in different tissues of the Micro-Tom cultivar showed that only the active homohexamer of GDH -subunits was detected in roots while heterohex- amers of GDH - and -subunits were found in fruits. These results indicate that GDH -subunit could modulate the heteromeric isoforms of GDH in response to the environment and physiology of the tomato fruit. This information is relevant to manipulate glutamate contents in tomato fruits genetically.