INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
CUESTAS ML,; MATHET, VL; JOSE OUBINA
JOURNAL OF VIRAL HEPATITIS.
WILEY-BLACKWELL PUBLISHING, INC
Lugar: Londres; Año: 2012 vol. 10 p. 754 - 754
PCR detection of viral genomes has provided new insights in viral diagnosis. Nowadays, it is the most frequently used Nucleic Acid Testing qualitative and quantitative technique. The aim of this study was to analyze the major circulating HBV variants PCR-amplified by three sets of primers in a patient infected with genotype E. HBV S/Pol overlapping genomic region was amplified from the serum of an infected child by using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol ?conferring resistance to Adefovir? could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be referred together with the sequence of the primers used for PCR amplification when studies of escape and / or antiviral resistant HBV mutants are carried out.