IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Analysis of the structure and surfactant activity of formulations containing pulmonary surfactant and antibiotics.
Autor/es:
LAIRION F; CIMATO, A; REPETTO M,; DE GROSSI J ; MARTÍNEZ SARRASAGUE M
Reunión:
Congreso; XLIX Reunión Anual de la Sociedad Argentina de Biofísica (SAB) 2021; 2021
Institución organizadora:
Sociedad Argentina de Biofísica (SAB)
Resumen:
Inhaled antibiotics (Atb) allow the delivery of high drug concentrations into the lungs and reduce systemic toxicity. Nowadays, local administration of Atb in the lung is only possible by nebulization of intravenous formulations, with undesirable side effects. The incorporation of Atb in the membranes of an exogenous pulmonary surfactant (EPS) could be a novel alternative to avoid the side effects and improve drug delivery. EPS would act as a carrier with therapeutic effect, so it is essential that Atb s incorporated into the EPS do not affect its biophysical properties.Objectives: To study the effects of Gentamycin (Genta), Amphotericin (Amph), Itraconazole (Itra), and Levofloxacin (Levo) on the structure and biophysical properties of EPS. Methods: EPS alone or added with each Atb were labeled with nitroxide spin labels to study the fluidity (order parameter S) and the lateral structure (S/W ratio) of the EPS membrane by Electron Spin Resonance. The EPS macrostructure (heavy and light subtypes), the amount of Atb incorporated in the EPS membranes and the tensioactive properties (ST) were also analyzed. Results: Amph and Itra were incorporated into EPS bellow 1 and 5 %m/m respectively and Levo was incorporated until 20%m/m. Genta is an hydrosoluble Atb, so did not incorporated to EPS membranes. Only Genta altered EPS macrostructure increasing the % of heavy subtype.Genta 10% and 20%m/m and Amph 3%m/m increased membrane fluidity (p