Voluntary running wheel effect in trx1-overexpression mice subjected to isquemia/reperfusion injury

PEREZ, V; YANAJE CY; CICALE E; BUCHHOLZ B; GODOY E; CASANOVA V; LAGO N; D'ANNUNZIO V; FRANCO RIVEROS, V; OSSANI G; GRECO MC; GELPI RJ

Virtual

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2021

Exercise training reduces myocardial injury caused by acute ischemia/reperfusion (I/R). Thioredoxin-1 (Trx1) maintains the cellular redox status and decreases the infarct size in I/R injury. However, it is not fully understood its role in exercise mice. The aim was to study if Trx1is involved in the exercise cardioprotection mechanism. Wild type mice hearts (Wt), transgenic mice hearts overexpressing Trx1, and a dominant negative mutant (DN) of Trx1 were used, mice were divided in exercise group (E) and sedentary group (S). Mice were placed in cages fitted with running wheels during 4weeks. After the exercise-training period, mice hearts were subjected to 30min of I and 120min of R (Langendorff technique). The assessment of the infarct size was performed using TTC. Also, heart rate variation (∆HR%) and mice, heart, and soleus weight was measured. Transverse muscle sections of soleus muscles were stained with H&E and the cross-sectional area (CSA) of myofibers were measure. Data were expressed as mean±SEM and p