NATRIURETIC PEPTIDES AND RENAL INFLAMMATION IN TWO MODELS OF HYPERTENSION: DEOXYCORTICOSTERONE ACETATE-SALT AND RENOVASCULAR 1 KIDNEY-1 CLIP

KOUYOMDZIAN N; ROBESAUL GD; FERNANDEZ B; RODRIGUEZ FERMEPIN M; YUN M; CHOI M; CAO G; DONATO M

Buenos Aires

Congreso; LXVI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2020

Sociedad Argentina de Investigación Clínica

Objectives: The aim was to demonstrate the hypothesis that in DOCA-salt (DS) and renovascular 1K-1C (RV) hypertensive models, the development of hypertension and the changes in natriureticpeptides (NP) secretion could be related to simultaneous over expression of NP receptors and inflammatory and fibrotic biomarkers in kidney.Materials: Male Sprague-Dawley rats were randomly divided in three groups: Control (C), DS (30 mg/kg-Salt 1% W/W and RV. After 6 and 12 weeks, systolic blood pressure (SBP) was measured bythe tail cuff method; plasma ANP and BNP levels were determined by commercial radioimmunoassay; mRNA expression of receptors NPR-A and NPR-C were measured in kidney by RT-PCR; and immunoexpression of inflammatory biomarkers (IL-6, TNF-α and NF-κB) and fibrotic biomarker TGF-β, by immunohistochemistry. Statistical analysis was performed by two-way ANOVA followed by a Tukey-Kramer test.Results: SBP increased in DS and RV at 6 and 12 weeks (mmHg: C6:118+2; DS6:194+2*; RV6:184±2*; C12:121±1; DS12:193±4*; RV12:203±4*). Plasma ANP increased in DS and RV (pg/mL: C6:170+68; DS6:689+143*; RV6:387±90*; C12:87±39; DS12:609±38*; RV12:294±57*), while plasmaBNP rose only in RV (pg/mL: C6:39±6; RV6:99±8*; C12:42±7; RV12:94±10*). mRNA levels of NPR-A (AU, C6:0.096±0.011; DS6:0.232±0.015*; C12:0.092±0.020; DS12:0.328±0.039*) and NPR-C (AU, C6:0.184±0.020; DS6:0.721±0.131*; C12:0.131±0.026; DS12:0.302±0.039*) increased only in DS. All biomarkers increased in DS at 6 and 12 weeks (*), while TGF-β and TNF-α enhanced in RV only at 12 weeks (*p