oxLDL-induced inflammation in isolated aorta: effects of short chain fatty acids.

PRINCE, PAULA D.; HID, EZEQUIEL J.; FRAGA, CESAR G.; LITTERIO, MARÍA C.; GALLEANO, MONICA

Modalidad virtual

Congreso; Reunión Anual de Sociedades de Biociencia SAIC. SAI. SAFIS. 2020; 2020

SAIC, SAI, SAFIS

Oxidized low density lipoproteins (OxLDL) has been implicated inNLRP3 inflammasome activation in the vascular wall. Short-chainfatty acids (SCFA) produced by gut microbiota, could provide beneficialeffects in attenuating endothelial dysfunction and inflammatoryresponse associated with metabolic diseases.The aim of this workwas to evaluate select short chain fatty acids (SCFA) on the Ox-LDL-induced NLRP3 activation in aorta by using an ex-vivo model.Thoracic aortas from male Sprague Dawley rats were cut in rings,and incubated with OxLDL (0-200 mg/ml) or vehicle for 0-120 min.At the end of the incubation periods, expression of proteins (NLRP3,procaspase-1, caspase-1, pro-IL1beta, TNFalpha) and detection of4-hidroxinonenal adducts (4HNE) were measured by western blot inthe aorta rings, and interleukin levels (IL1beta and IL18) by ELISAin the incubation media. OxLDL produced modifications in all themeasured parameters, with different time-course and degree of response.The earliest responses (10 min) were observed for NLRP3expression in aorta (increments of 25, 125, and 100% for 50, 100and 200 mg/ml OxLDL, respectively p